Previous research in the world, as well as in the Republic of Serbia, was aimed at researching the impact of (not) using daytime running lights, in passenger vehicles, on traffic safety. Research on the impact of the (non) use of daytime running lights by motorcycles on traffic safety has been scarce. Bearing the above in mind, as well as the fact that the occurrence of traffic accidents is influenced by many factors, the authors of this paper decided to conduct an experimental study on a driving simulator, with the aim of determining how (not) the use of daytime running lights on motorcycles affect the estimation of the speed of the motorcycle (30 km/h, 50 km/h, 70 km/h, 90 km/h). 128 young drivers participated in the research. The results of the research showed that there are statistically significant differences in the estimation of the speed of the motorcycle by road users when the daytime running lights are on and when the daytime lights are not on, for all tested speeds and in all tested conditions.