Banja Luka : University of Banja Luka, Faculty of Agriculture
Influence of the Macrophomina phaseolina (Tassi) Goid. on SOD activity in four different genotypes of soybean seedlings
2021
Macrophomina phaseolina (Tassi) Goid. is a very common pest in Serbia, which
affects root and lower stem in many plants, causing charcoal rot. Among other plants,
soybean is also often affected by this pathogen and, like others, on the attack
responds with oxidative burst. The aim of this work was to compare the sensitivity
of four different genotypes of soybean (Glycine max (L.) Merr.): FAVORIT,
ATLAS, SAVA and RUBIN on this pathogen. The activity of antioxidant enzyme
superoxide-dismutase (SOD; EC 1.15.1.1.) was compared between four soybean
genotypes, after the attack of the fungus M. phaseolina. Soybean seeds were
inoculated using the method of artificial inoculation on filter paper. After seven days,
seedlings were collected for biochemical assays. It was measured 1 g of plant
material fresh weight and homogenized with 10 ml 0.1 M K2HPO4, pH 7.0. After
centrifugation, the supernatant was used for enzyme activity measurements. The
SOD activity was measured spectrophotometrically, by monitoring the inhibition of
nitroblue tetrazolium (NBT) reduction at 560 nm, according to method by Mandal et
al (2008)
Banja Luka : University of Banja Luka, Faculty of Agriculture
Influence of the Macrophomina phaseolina (Tassi) Goid. on SOD activity in four different genotypes of soybean seedlings
Macrophomina phaseolina (Tassi) Goid. is a very common pest in Serbia, which
affects root and lower stem in many plants, causing charcoal rot. Among other plants,
soybean is also often affected by this pathogen and, like others, on the attack
responds with oxidative burst. The aim of this work was to compare the sensitivity
of four different genotypes of soybean (Glycine max (L.) Merr.): FAVORIT,
ATLAS, SAVA and RUBIN on this pathogen. The activity of antioxidant enzyme
superoxide-dismutase (SOD;...
Preuzmite dokument
2021
Crnković, Marina
,
Šućur, Jovana
,
Petrović, Kristina
,
Malenčić, Đorđe
