The aim of this study was to determine the protein content of wheat grain in a sustainable production method. The research was conducted at the Scientific Institute PKB Agroekonomik Padinska Skela (2017-2019) with four varieties of wheat (Ratarica, Pobeda, Nogal and Apache) in densities of 400 and 500 grains/m2. The basic fertilization was with 400 kg NPK/ha. Feeding was performed: T1 with 150 kgN/ha; T2 150 kgN/ha+1 foliar treatment with multiple inoculum T3 100 kgN/ha+2 foliar treatments and T4 50 kgN/ha+3 foliar treatments. The multiple preparation (EM Aktiv 6 l/ha) is a large group of effective microorganisms